cell growth medium mv low serum Search Results


96
Cell Applications Inc hc growth supplement
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Cell Applications Inc porcine endothelial cell growth medium pecgm
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Celprogen Inc stem cell growth medium
Stem Cell Growth Medium, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CELLnTEC Advanced Cell Systems AG serum-free keratinocyte medium supplemented bovine pituitary extract epidermal growth factor
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European Collection of Authenticated Cell Cultures dulbecco’s modified eagle’s medium supplemented with 10% fetal bovine serum and antibiotics (growth medium)
Dulbecco’s Modified Eagle’s Medium Supplemented With 10% Fetal Bovine Serum And Antibiotics (Growth Medium), supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cellgro gmp serum-free stem cell growth medium scgm
Gmp Serum Free Stem Cell Growth Medium Scgm, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lifeline Cell Technology serum-free keratinocyte growth medium (kgm)
Serum Free Keratinocyte Growth Medium (Kgm), supplied by Lifeline Cell Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cambrex human mscs between passages 4 and 10
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Cellgro serum-free stem cell growth medium scgm
Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, <t>SCGM</t> and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.
Serum Free Stem Cell Growth Medium Scgm, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Lonza endothelial cell growth medium containing 2% fetal bovine serum (fbs)
Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, <t>SCGM</t> and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.
Endothelial Cell Growth Medium Containing 2% Fetal Bovine Serum (Fbs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial cell growth medium containing 2% fetal bovine serum (fbs)/product/Lonza
Average 90 stars, based on 1 article reviews
endothelial cell growth medium containing 2% fetal bovine serum (fbs) - by Bioz Stars, 2026-04
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90
Cellgro gmp serum-free stem cell growth medium
Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, <t>SCGM</t> and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.
Gmp Serum Free Stem Cell Growth Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gmp serum-free stem cell growth medium/product/Cellgro
Average 90 stars, based on 1 article reviews
gmp serum-free stem cell growth medium - by Bioz Stars, 2026-04
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Image Search Results


Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, SCGM and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.

Journal: PLoS ONE

Article Title: Derivation of Xeno-Free and GMP-Grade Human Embryonic Stem Cells – Platforms for Future Clinical Applications

doi: 10.1371/journal.pone.0035325

Figure Lengend Snippet: Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, SCGM and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.

Article Snippet: In the next step, we identified a commercially available serum-free stem cell growth medium (SCGM; CellGro®/CellGenixTM) which is designed for the expansion of hematopoietic and natural killer cells.

Techniques: Derivative Assay, Cell Culture, In Vitro, Immunofluorescence, Staining, Expressing, Recombinant, Activity Assay