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Image Search Results
Journal: PLoS ONE
Article Title: Derivation of Xeno-Free and GMP-Grade Human Embryonic Stem Cells – Platforms for Future Clinical Applications
doi: 10.1371/journal.pone.0035325
Figure Lengend Snippet: Feeders derived from the three types of human tissues were equally effective in maintaining undifferentiated pluripotent growth of hESCs (HES-1 ) for a minimum of 5 passages within research-grade KO medium. (A) The hESCs cultured on the three types of feeders formed colonies with typical morphology (phase contrast images), expressed alkaline phosphatase (AP), and were immunoreactive with anti-Oct-4 (nuclei were counterstained with DAPI). They were pluripotent as demonstrated by their potential to differentiate in-vitro into progeny representing the three germ lineages. Immunofluorescence staining showed differentiated cells expressing beta-tubulin III (ectoderm), muscle actin (mesoderm) and alpha-Feto-Protein (AFP, endoderm). (B) FACS analysis showing that the percentage of cells expressing markers of pluripotent human stem cells, and the level of background differentiation (percentage of SSEA-1 expressing cells) was similar (n = 3). (C) The hESC doubling time did not differ significantly after culture on the three types of feeders (n = 3). Cord feeders were further used for the development of the clinical grade culture system, and the phenotype of hESCs was compared after culture within research-grade KO medium system and following replacement and supplementation with xeno-free and GMP-grade reagents. The following culture compositions were compared: hESCs cultured on porcine gelatin (porc-gel) or in recombinant gelatin (rec-gel) in KO medium, SCGM and SCGM supplemented with HSA (SCGM-HSA). (D) With all culture conditions, the hESCs colonies had similar and typical morphological characteristics (phase contrast images), and the stem cells expressed alkaline phosphatase activity (red) and Oct-4 (green; blue-DAPI nuclear counterstaining; immunofluorescence images). (E) The percentage of cells expressing Tra-1-60 and Tra-1-81 was analyzed by FACS and compared between the various culture conditions (n = 4). Scale bar in (D) represents 200 um.
Article Snippet: In the next step, we identified a commercially available
Techniques: Derivative Assay, Cell Culture, In Vitro, Immunofluorescence, Staining, Expressing, Recombinant, Activity Assay